Treg Expansion Kit, human
Description The Treg Expansion Kit is designed for the efficient expansion of Treg cells. Using this kit FoxP3 expression by Treg cells is sustained after isolation with the CD4+CD25+CD127dim/– or CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs.1 The kit is also suitable for usage with rapamycin.2 The Treg Expansion Kit is based on MACSiBead™ Particles pre‑loaded with CD3 and CD28 antibodies. Optimal expansion of Treg cells is accomplished by using pre-loaded MACSiBead Particles and Treg cells at a bead-to-cell ratio of 4:1 and recombinant interleukin 2 (rIL-2) with a concentration of 500 U/mL. Applications Expansion of human Treg cells after isolation with the CD4+CD25+CD127dim/– or CD4+CD25+CD45RA+ Regulatory T Cell Isolation Kits from human blood or PBMCs. Expanded Treg cells can be used for any downstream application such as cytokine analysis, gene expression, and suppression assays.		Figure 1 Treg cells were isolated from human PBMCs by using the CD4+CD25+CD127dim/– Regulatory T Cell Isolation Kit II and expanded with the Treg Expansion Kit for 14 days. Treg cells before (A,B) and after expansion in the absence (C,D) or presence (E,F) of rapamycin were fluorescently stained and analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to CD4 expression. A: Isolated Treg cells B C: Expanded Treg cells (without rapamycin) D E: Expanded Treg cells (with rapamycin) F Figure 2 Expansion rates of Treg cells were calculated from the numbers of FoxP3-expressing cells before and after 14 days of expansion in the absence or presence of rapamycin.

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