CMV IE-1 – Recombinant Protein
Description Human cytomegalovirus (CMV) is a member of the herpes virus group belonging to the subfamily of beta-herpes viruses. Between 50–85% of human adults are infected with CMV. Once infected, the virus persists in the organism. The infection is asymptomatic in healthy individuals, but in immunocompromised patients CMV can cause severe diseases. CMV IE-1 (immediate early protein 1), also known as UL123, is a non-structural protein, which is one of the first CMV antigens expressed in an infected cell, and predominantly induces a CD8+ T cell response. IE-1–specific T cells occur in infected individuals at frequencies comparable to those of pp65-specific CD8+ T cells.¹ Both CMV antigens, IE-1 and pp65, are considered to be dominant T cell targets. Applications CMV IE-1 – Recombinant Protein is specially developed for efficient loading of monocyte derived dendritic cells (Mo-DCs) for subsequent restimulation of IE-1–specific CD4+ and CD8+ T cells. The effector cytokines produced after stimulation allow identification and analysis of IE-1–specific T cells.		106 human T cells from a CMV+ donor were restimulated for 6 hours using unloaded in vitro generated DCs or in vitro generated DCs loaded with CMV IE-1 – Recombinant Protein. After 2 hours of restimulation 1 μg/mL of brefeldin A was added. Cells were harvested, fixed, permeabilized, and IE-1–specific cells were intracellularly stained with Anti-IFN-γ-PE. T cells were counterstained with CD8-FITC. IFN-γ production of lymphocytes is shown. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Stimulated cells Unstimulated control

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