miRXplore™ Universal Reference
miRXplore™ Universal Reference for microRNA research The miRXplore™ Universal Reference pool was developed to meet microRNA researchers’ demand for a reliable, comprehensive microRNA reference tool. Thus, the miRXplore Universal Reference is an equimolar pool of more than 950 single-stranded synthetic RNA oligonucleotides matching mature microRNAs2. For microRNA microarray experiments, the miRXplore Universal Reference can be used as a: • Reference • Hybridization control • Quality control for microarrays • Positive control for microRNA cloning experiments • Absolute quantification of microRNAs1 Also, the reference can be used for real-time PCR applications, for example, as positive control and quantification tool1+2. Usage of miRXplore Universal Reference Comparison of multiple samples The miRXplore Universal Reference can be used to compare multiple samples and can help to save sample material. The reference comprises all human, mouse, rat, and virus microRNAs according to the miRBase Sequence Database version 9.2 released by the Sanger Institute3. Features of miRXplore Universal Reference • The miRXplore UR is available in two product formats containing either 25 or 125 fmol per microRNA. • Unmodified, HPLC-purified synthetic RNA oligonucleotides that were controlled by mass spectroscopy and PAGE analysis. Concentration of each oligonucleotide was measured by at least two methods before adding equimolar amounts (5 fmol). Disclaimer This product and its use are covered by one or more patents owned by Oxford Gene Technology Limited or Oxford Gene Technology IP Limited (togehter "OGT"): US 6, 054, 270; EP 0, 373, 203; JP 3, 393, 528 and 3, 386, 391 and pending patents. The purchaser is licensed to practise methods and processes covered by these patents using this product, but may not: transfer data derived from the use of this product to third parties for value; use this product to make, have made, create, or contribute to the creation of stand-alone expression database product for license, sale, or other transfer to a third party for value; or use this product for the identificaton of antisense reagents or the empirical design of probes or sets of probes for using or making nucleic acid arrays. Further information miRXplore™ Microarray flyer [PDF; 367 KB] miRXplore™ Micorarray Kit [PDF; 651,3 KB] Universal reference for miRNA research application note [PDF; 119,4 KB]		Figure 1 Example of miRXplore™ Universal Reference (UR) application. Direct comparison of liver versus brain and indirect comparison of liver or brain versus miRXplore UR, respectively. A overlay scan, B cluster analysis. Figure 2 Doubled signal detection sensitivity in microRNA profiling experiments using the automated a-Hyb™ Hybridization Station. Comparison of automated versus manual hybridizations using the a-Hyb Hybridization Station (left) versus static cover slip hybridization (right) in microRNA profiling experiments.

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