T Cell Activation/Expansion Kit, mouse
Description The T Cell Activation/Expansion Kit is designed to activate and expand mouse T cells. The kit consists of Anti-Biotin MACSiBead™ Particles and biotinylated antibodies against mouse CD3ε and CD28. Anti-Biotin MACSiBead Particles loaded with biotinylated antibodies are used to mimic antigen-presenting cells and activate resting T cells in single-cell suspensions from spleen or lymph nodes as well as purified T cells. T cell expansion is achieved by culturing and reactivation at day 6–8 of culture. Applications The T Cell Activation/Expansion Kit, mouse, can be used for activation and expansion of resting mouse T cells from splenocytes, or for activation and expansion of T cells isolated with MACS® Separation Reagents for mouse T cells.		Anti-Biotin MACSiBead Particles were loaded with CD3ε-Biotin and CD28-Biotin. Mouse spleen cells were activated for 20 hours, using one loaded Anti-Biotin MACSiBead Particle per lymphocyte. Cells were kept in RPMI and 10% FCS, without rIL-2. The negative control experiment was performed without adding MACSiBead Particles. Cells were fluorescently stained using CD4, CD8, and CD25 antibodies. CD4+ T cells stimulated unstimulated CD8+ T cells stimulated unstimulated

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