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| Description |
The CD8 antibody recognizes the human CD8 antigen which is strongly expressed on human cytotoxic T cells and thymocytes, and is also expressed on a subset of NK cells. The CD8 antigen is a disulfide-linked dimer that exists either as a CD8α homodimer or as a CD8α/β heterodimer. CD8 acts as a coreceptor for the T cell receptor and binds to the MHC Class I molecule.
The CD8 antibody recognizes the α-subunit of the antigen.
The VioBlue-conjugated CD8 antibody requires the use of a violet laser. |
| Applications |
The CD8 antibody can be used to identify and enumerate CD8+ cells by flow cytometry or fluorescence microscopy.
This antibody is suitable for MACS® Control applications. For further details, please refer to the data sheet of the separation reagent. |
| Cross-reactivity |
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| BW135/80 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD8 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue™ (D), or PerCP (E), and analyzed by flow cytometry. Cells were gated on viable lymphocytes. |
| A |
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| B |
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| C |
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| D |
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| E |
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