CMV pp65 – Recombinant Protein
Description Human cytomegalovirus (CMV, HCMV) is a member of the herpes virus group and belongs to the subfamily of beta-herpes viruses. Between 50 and 85% of human adults are infected with CMV. Once infected, the virus persists in the organism. The infection is asymptomatic in healthy individuals but in immunocompromised patients CMV can cause severe disease. The pp65 protein (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. pp65 is an immunodominant target of CD4+ as well as CD8+ T cell responses to CMV. pp65-specific T cells predominantly produce cytokines, such as IFN-γ, IL-2, and TNF-α. Applications Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals. The in vitro stimulation of pp65-specific CD4+ and CD8+ T cells with CMV pp65 – Recombinant Protein causes the secretion of effector cytokines. These cytokines then permit pp65-specific effector/memory T cells to be detected, for example, by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies. CMV pp65 – Recombinant Protein can also be used to restimulate cells for the isolation of viable pp65-specific CD4+ and CD8+ T cells, for example, with the use of MACS® Cytokine Secretion Assay – Cell Enrichment and Detection Kits, or for the generation of pp65-specific CD4+ and CD8+ T cells from naive T cell populations, for example in immunotherapy or vaccination research. The pulsing of antigen-presenting cells, for example, dendritic cells with CMV pp65 – Recombinant Protein can also be used as a model in research on antigen uptake and presentation by such cells.		Figure 1 Human PBMCs of a CMV+ donor were restimulated for 6 hours with or without CMV pp65 – Recombinant Protein. Brefeldin A was added after 2 hours. Cells were fixed, permeabilized, and pp65-specific cells were intracellularly stained with Anti-IFN-γ-PE. T cells were counterstained for CD4 and CD8 expression; IFN-γ production of lymphocytes is shown. CD4+ T cells A: Stimulated sample B: Unstimulated control CD8+ T cells C: Stimulated sample D: Unstimulated control

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