Whole Blood CD56 MicroBeads
Description Whole Blood CD56 MicroBeads were developed for rapid positive selection of CD56+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD56 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit. The CD56 antigen is expressed by most NK cells and a T cell subset. Upon activation of NK cells, the surface expression of CD56 is increased. Applications Whole Blood MicroBeads allow the fast isolation of CD56+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yield of target cells. Whole Blood MicroBeads in combination with the autoMACS™ Separators allow standardization of the cell separation procedure and safe handling of hazardous samples. The purified CD56+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2. For more information on MACS® Whole Blood MicroBeads click here. Columns autoMACS Columns: autoMACS™ Pro Separator (Program Posselwb; 0.25–15 mL sample volume) or autoMACS Separator (Program Posseld2; 0.25–3 mL sample volume) Whole Blood Column Kit: for 0.25–15 mL sample volume		Figure 1 CD56+ cells were separated from whole blood using Whole Blood CD56 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were stained with CD56-PE and CD3-FITC.

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