Whole Blood CD4 MicroBeads
Description Whole Blood CD4 MicroBeads were developed for rapid automated positive selection of CD4+ cells directly from whole blood or bone marrow. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. Anticoagulated cell samples of 0.25–15 mL are labeled with Whole Blood CD4 MicroBeads to be subsequently separated with the autoMACS™ Pro Separator or the autoMACS Separator, or using the Whole Blood Column Kit. Whole Blood CD4 MicroBeads recognize about 65% of all peripheral blood T cells. The CD4 antigen is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 antigen is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimer on CD4+ T helper cells. Applications Whole Blood MicroBeads allow the fast isolation of CD4+ cells directly from whole blood or bone marrow, thus minimizing hands-on time and maximizing yields of target cells. Whole Blood MicroBeads in combination with the autoMACS™ Separators allow standardization of the cell separation procedure and safe handling of hazardous samples.. The purified CD4+ cells are well-suited for further flow cytometric, functional, or molecular analysis including lineage-specific chimerism analysis after allogeneic stem cell transplantation1,2. For more information on MACS® Whole Blood MicroBeads click here. Columns autoMACS Columns: autoMACS™ Pro Separator (Program Posselwb; 0.25–15 mL sample volume), or autoMACS Separator (Program Posseld2; 0.25–3 mL sample volume) Whole Blood Column Kit: for 0.25–15 mL sample volume		Figure 1 CD4+ cells were separated from whole blood using Whole Blood CD4 MicroBeads and the autoMACS™ Separator with program Posseld2. Cells were fluorescently stained with CD4-PE and CD14-FITC.

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