| Description |
The Naive CD4+ T Cell Isolation Kit II provides a fast and efficient way to isolate untouched naive CD4+ T cells from human peripheral blood. It is the new generation of the Naive CD4+ T Cell Isolation Kit, for even higher purities of the isolated naive T helper cell population.
For magnetic depletion of memory CD4+ T cells and non-CD4+ T cells, PBMCs are incubated with a cocktail of biotinylated CD45RO, CD8, CD14, CD15, CD16, CD19, CD25, CD34, CD36, CD56, CD123, anti-TCRγ/δ, anti-HLA-DR, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion.
This product replaces the Naive CD4+ T Cell Isolation Kit, human (#130-091-894). |
| Applications |
Naive CD4+ T cells isolated with the Naive CD4+ T Cell Isolation Kit II can be used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on naive T helper cell activation and in functional studies of DCs.1, 2 Untouched naive CD4+ T cells can also be used for further isolation of specific T helper cell subsets.
For isolation of naive CD4+ recent thymic emigrants, click here. |
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| Figure |
| Isolation of untouched naive CD4+ T helper cells from PBMCs using the Naive CD4+ T Cell Isolation Kit II, an LS Column, and a MidiMACS™ Separator. Cells were stained with CD4 and CD45RA antibodies. |
| Before separation |
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| Isolated untouched naive CD4+ T cells |
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