Inside Stain Kit
Description The Inside Stain Kit is a unique tool for intracellular staining of cells in suspension, cells on slides, or cells immobilized on a MACS® Separation Column. For the latter protocol, target cells are magnetically enriched on an MS Column according to the expression of a chosen antigen. After elution, the cells are fixed with Inside Fix and directly applied onto a second column. Permeabilization, intracellular staining, and washing steps are performed whilst the cells are retained on the column. The stained cells are eluted and can subsequently be analysed by immunocytochemistry or flow cytometry. This approach is easy to perform, requires small volumes of staining reagents, and cell loss is minimal compared to conventional staining techniques. Thus, the Inside Stain Kit is an ideal tool, for example for the detection of enriched tumor cells. The Inside Stain Kit contains reagents for up to 50 tests. Applications Immunocytochemical detection and analysis of enriched epithelial cells based on the expression of intracellular antigens, for example, cytokeratins. Analysis of clonality of enriched B cell lymphoma or myeloma cells by staining for intracellular immunoglobulin light chains. Intracellular cytokine staining in pre-enriched T cells, dendritic cells, etc. See also: MACS® Cell Analysis Columns Optimal staining can be achieved using MS Columns		Figure 1 In-column intracellular staining of cells isolated with MACS® Technology.

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