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| Description |
| MACS® HSC-CFU Media have been developed for the enumeration of human hematopoietic stem and progenitor cells as Colony Forming Units (CFU). These standardized semi-solid media are based on methylcellulose in IMDM, supplemented with FBS, BSA, and different growth factors (e.g. GM-CSF, G-CSF, SCF, IL-3, IL-6, and Epo). The semi-solid MACS® HSC-CFU media are designed to maximize growth and differentiation of progenitor cells and to allow the clonal progeny of a single cell to grow in a distinct cluster or colony. MACS HSC-CFU media are produced under tightly controlled manufacturing conditions by using highly qualified raw materials to provide a consistent and optimally performing colony assay. |
| Applications |
HSC-CFU assays in methylcellulose-based, semi-solid media are the gold standard method for analyzing the hematopoietic engraftment potential of:
- separated hematopoietic stem cells (HSCs, e.g. CD133+ and CD34+ cells),
- unseparated blood, bone marrow, or fetal liver cells,
- ESC- or iPS cell–derived HSCs.
The HSC-CFU complete with Epo medium supports the growth of granulocyte colonies (CFU-G), macrophage colonies (CFU-M), granulocyte/macrophage colonies (CFU-GM), erythroid colonies (BFU-E and CFU-E), and mixed colonies (CFU-GEMM).
The HSC-CFU complete w/o Epo medium supports the growth of granulocyte colonies (CFU-G), macrophage colonies (CFU-M), and granulocyte/macrophage colonies (CFU-GM).
The HSC-CFU lite with Epo medium supports the growth of granulocyte colonies (CFU-G), macrophage colonies (CFU-M), granulocyte/macrophage colonies (CFU-GM), erythroid colonies (BFU-E and CFU-E), and mixed colonies (CFU-GEMM).
The HSC-CFU basic medium permits cytokines and other factors of choice to be added, thus providing the flexibility needed for specific research purposes. This formulation does not contain erythropoietin or other cytokines.
MACS HSC-CFU Media were used for various applications.1–4 |
| Certificate of Analysis |
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| Figure 1 |
| MACS® HSC-CFU Media. |
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| Figure 2 |
| Formulation of HSC-CFU media. |
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| References |
| 1. Del Fante et al. (2005) Stem Cells Dev. 14: 734–739. |
| 2. Chang et al. (2006) Blood 108: 1515–1523. |
| 3. Biedermann et al. (2007) Leuk. Res. 31: 211–220. |
| 4. Watts et al. (2008) Cytotherapy 10 (suppl. 1): poster no. 150. |
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