μMACS™ and MultiMACS™ Protein A or Protein G Kits
IP, Co-IP or ChIP of virtually any protein The µMACS™ and MultiMACS™ Protein A/G Kits were developed for analytical scale immunoprecipitation (IP), Co-IP, or chromatin-IP (ChIP) of proteins. The tiny small (diameter 50 nm), non-sedimenting µMACS™ Protein A/G MicroBeads ensure very rapid reaction kinetics and the formation of the labeled immune complex is generally completed in 30 minutes. High speed MACS® Technology saves time. The experiment can be completed in less than 2 hours, while conventional IPs usually require overnight incubation steps and last up to one day. High specificity The low non-specific binding of µMACS Protein A/G MicroBeads and the efficient and gentle washing in the column significantly reduce background (fig. 2). The washing procedure can be optimized for any target, and even fragile protein complexes can be successfully isolated by co-IP (fig. 3) with MACS Technology. High sensitivity Due to the fast reaction kinetics of the small, non-sedimenting µMACS MicroBeads, binding to target proteins is extremely fast and efficient, resulting higher amount of target proteins. Even rare proteins can be efficiently isolated. ChIP-in-a-column Chromatin immunoprecipitation6 (ChIP) protocols also benefit from the higher specificity and lower background of µMACS Protein A/G MicroBeads. Please click here and download a ChIP customer report. For a detailed ChIP protocol based on MACS Technology please click here. Low- to high-throughput applications The µMACS Protein A or G MicroBeads were developed for manual, low-throughput applications with the µMACS Separator. The procedure can easily be upscaled with the MultiMACS Protein A or G Kit to a semi- or fully-automated, high-throughput processing of up to 96 samples in parallel by utilizing the MultiMACS 96 Separator.20 Columns For µMACS™ Protein A or G MicroBeads: µ Column For MultiMACS™ Protein A or G Kit: Multi-8 or Multi-96 Columns (included in the kits) Further information Chromatin immunoprecipitation (ChIP) [PDF; 94 KB]		Figure 1 Overview: immunoprecipitation using μMACS™ Protein A or Protein G MicroBeads. Figure 2 Immunoprecipitation of the SV40 large T antigen from COS cells using μMACS Protein G MicroBeads. Silver stained SDS gel of the cell lysate (L), a protein marker (M), the immunoprecipitated large T antigen (lane 1, indicated by the arrow), an isotype-matched control antibody (2, immunoprecipitation with rat anti-mouse antibody), and a control without antibody (3) are shown. Figure 3 Detection of biotinylated surface antigens of the B cell line JOK-I following immunoprecipitation. For immunoprecipitation anti-CD22 (HD239, lane 2) or anti-MHC class I (W6/32, lane 4) antibodies were used. An isotype-matched antibody (1, 3) was used as a control. Following SDS-PAGE and transfer to a membrane, biotinylated proteins were detected using streptavidin-conjugated peroxidase. (Courtesy of Dr. Reinhard Schwartz-Albiez, Heidelberg, Germany.) Figure 4 Co-immunoprecipitation of beta-catenin (BCat): Androgen receptor was immunoprecipitated from dihydrotestosterone (DHT)-stimulated (lane 1, 3) or unstimulated LNCaP cells (2, 4) with µMACS Protein G MicroBeads (1, 2) or with Protein A/G agarose beads (lanes 3, 4 ). Western blot (WB) using anti-beta-catenin antibody shows Beta-Catenin co-immunoprecipitated with androgen receptor. (Courtesy of D. Mulholland, Vancouver, Canada)

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