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Target MSC populations with accuracy
To investigate the therapeutic potential of MSCs it is crucial to establish efficient and reproducible procedures to obtain homogenous MSC populations. Miltenyi Biotec is a long-standing provider of high-quality kits and reagents for the separation of MSCs from different species using a plethora of markers.
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Isolate MSCs from various tissues with ease using MACS Technology. Be assured that isolated MSC populations retain their high proliferation and differentiation potential in vitro. For example, MSCs separated with the CD271 MicroBead Kit display a 10-100–fold higher proliferative capacity than cells isolated by plastic adherence alone.
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MSCs are often heterogeneous populations that are contaminated by lympho-hematopoietic cells, hematopoietic stem cells, and macrophages. Therefore, the isolation of MSCs can be a difficult task. Mouse MSCs can be efficiently isolated by MACS Technology in the same way as any other cell type that expresses a specific cell surface antigen. A wide range of MicroBeads are available for the enrichment or purification of mouse MSCs with a high yield and purity. Various approaches for the separation of MSCs from other cells can be utilized:
- Mouse MSCs expanded from bone marrow cultures can be enriched by selection with CD105, CD117, or Anti-Sca-1 MicroBeads
- Multipotent plastic-adherent fetal stem cells can be enriched from amniocentesis cultures according to their expression of CD117
- aminating cells can be depleted from mouse MSC cultures by the use of CD11b MicroBeads or a combination of CD45 and Anti-Ter-119 MicroBeads
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Are you utilizing a large animal experimental model? Expand your experimental possibilities with the CD271 antibody, which cross-reacts with non-human primate, bovine, swine, and canine MSCs.
If the reagent of your interest is not included in the MACS® Cell Separation Reagent portfolio, you can simply use indirect magnetic labeling for customized cell separation. Indirect magnetic labeling is a convenient alternative to direct labeling as any primary antibody may be used for efficient MACS Separation.
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Discover full experimental flexibility with indirect magnetic labeling
- Anti-Fluorochrome MicroBeads for MACS Separations with fluorochrome-conjugated primary antibodies
- Anti-Biotin MicroBeads, Streptavidin MicroBeads for MACS Separations with biotinylated primary antibodies
- Monoclonal or Polyclonal Anti-Ig MicroBeads for MACS Separations with unconjugated primary antibodies
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| September 2010 |
Neuroscience Ireland Annual Conference
September 2-3
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2010 World Molecular Imaging Congress
September 8-11
Kyoto, Japan
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