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Comprehensive solutions for cell culture
Choosing the right medium is critical for stem cell culture. With that in mind, we offer an expanding portfolio of proven MACS® and Stemgent® Media for the reliable culture of stem cells and standard eukaryotic cell lines. Our media, cytokines and growth factors have been optimized to promote healthy morphology and expression of pluripotent markers.
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The MACS Media and Stemgent product lines have joined forces to offer a comprehensive selection of ready-to-use cell culture media with a broad range of applications. All of our media are shipped with clear protocols to assist you in efficiently establishing optimal conditions for stem cell expansion and differentiation.
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We offer a range of cytokines, growth factors and related proteins where quality and convenience takes precedence. Our cytokines, for example, are available as research-grade, premium-grade and GMP-grade products – a product range extending from the bench to bedside. Moreover, each batch of premium- and GMP-grade cytokines are ready-to-use and have been tested for biological activity and endotoxin levels.
For a comprehensive list of available cytokines, visit Miltenyi Biotec’s global website.
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Small molecules may act by affecting key epigenetic modifications and cell signaling pathways and have been proven to be invaluable effectors of stem and iPS cells. Defined cocktails of small molecules can affect cell reprogramming, self-renewal, and differentiation.
Benefit from a unique portfolio of Stemgent® Small Molecules — potent tools that are available in the highest purity (≥98%), have been structurally verified by NMR and mass spectrometry, and have been tested for cytotoxicity.
Small molecules with convincing results:
- Easy cell penetration
- Very low antigenicity
- Work across multiple cell signaling pathways
- Easily affect epigenetic modifications
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With Stemgent’s tried and tested virus and plasmid sets for stem cell reprogramming, you can save time and effort from not having to generate and validate viruses in your own lab. With the addition of small molecules you can greatly enhance reprogramming efficiency.
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Stem cells require special conditions for survival and growth and an important aspect of this is the underlying matrix. The reconstituted basement membrane (RBM) isolated from the mouse Engelbreth-Holm-Swarm (EHS) tumor is a matrix composed of laminin, collagen IV, heparin sulfate, proteoglycan, nidogen, entactin, and growth factors that supports the attachment, growth, and differentiation of many adherent cell types. RBM is able to support the growth and maintenance of mouse and human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells.
Laminin, a glycosylated protein, is the main component of RBM. Laminin can serve as an alternative to mouse embryonic fibroblast (MEF) feeder cells for propagating pluripotent human or mouse cell lines and can be used to support derivation and differentiation of lineage-restricted progenitor cells. Also, pure laminin can support ES and iPS cell growth in the undifferentiated state, when it is desirable to have defined growth conditions. Stemgent® Laminin should be used as a cell attachment matrix when growth factors and other contaminants present in less pure RBM gels might compromise an experiment.
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Stemgent’s primary stem cell lines have been successfully used in many laboratories for use as experimental controls or for studying epigenetic modifications. Mouse primary iPS cells-T1b and -WP5 are induced pluripotent stem (iPS) cells that have not been genetically modified. When cultured under standard mouse ES cell conditions, they are morphologically identical to mouse ES cells. They also express the pluripotency marker SSEA-1 and have strong AP activity. Mouse primary iPS cells-NNeo harbor the reverse tetracycline transactivator (rtTA), Oct4 cDNA, a neomycin resistance gene, and the doxycycline-inducible transcription factors Klf4, Sox2 and c-Myc.
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Stem Cell Updates.
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| September 2010 |
Neuroscience Ireland Annual Conference
September 2-3
Dublin, Ireland |
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2010 World Molecular Imaging Congress
September 8-11
Kyoto, Japan
www.wmicmeeting.org |
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