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Benefit from the recognized standard in cell separation for ESC/iPSC isolation
To unravel the molecular mechanisms that govern the reprogramming, self-renewal and differentiation of ES and iPS cells, it is imperative to have an excellent source of homogenous cell populations. Isolate ES and iPS cells in a gentle and reliable fashion using MACS® Technology — the gold standard in cell separation. |
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ES and iPS cells are often cultured in the presence of supportive feeder cells that can interfere with downstream applications. Positive or negative selection with MACS® Technology can rapidly separate viable ES or iPS cells from co-cultures.
Labeling human cells
- Deplete (or enrich) human fibroblasts with Anti-Fibroblast MicroBeads
- Pluripotent Stem Cell (CD326) MicroBeads, human, to isolate human pluripotent cells
Labeling mouse cells
- Anti-SSEA-1 (CD15) MicroBeads allow the positive selection of undifferentiated, pluripotent mouse ES and iPS cells
- Feeder cell removal MicroBeads, mouse
Indirect labeling
- Using indirect magnetic labeling it is possible to deplete any type of feeder cell as long as they express a surface marker that is not expressed by the co-cultured cells
- Indirect labeling of SSEA-4 or SSEA-3 can be used to isolate human pluripotent cells from mixed populations
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| Dramatically enhance the efficiency of cell reprogramming by using homogeneous cell populations. Adult stem cell populations can be more efficiently reprogrammed compared to terminally differentiated cells. For example, it has been shown that CD34+ adult stem cells can be used for reprogramming. Moreover, CD133+ cells can be isolated from fresh or frozen cord blood before being reprogrammed to the pluripotent state. The utilization of neural stem cells is also beneficial as they already express Sox 2. |
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ES and iPS cells tend to differentiate in culture. However, it is crucial to obtain homogeneous pluripotent cultures for experiments like differentiation, gene expression, or microRNA studies. MACS Technology is a straightforward tool for the enrichment of pluripotent stem cells either by depletion of differentiated cells, or by positive selection according to the expression of pluripotency-associated markers, e.g., CD326, CD133, SSEA-3, SSEA-4, TRA-1-60, or TRA-1-81. Positive selection of cells according to these markers saves time and reduces the variability of results by ensuring that only pluripotent cells are utilized in downstream applications. These markers may also be used to deplete unwanted pluripotent cells from differentiated cultures.
- MicroBeads specific for SSEA-1 allow for the isolation or depletion of pluripotent cells from mouse cell cultures
- MicroBeads for indirect magnetic labeling allow for isolation or depletion of any cell type from any species
- Pluripotent Stem Cell (CD326) MicroBeads and Anti-Tra-1-60 MicroBead Kit for the positive isolation of pluripotent human cells
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ES and iPS cells can differentiate in vitro into virtually any cell type. MACS® Technology allows the rapid isolation of cells from specific lineages at any time point during differentiation. These cells express the same surface markers as their respective progenitors from adult tissue. MACS Technology can be used to deplete unwanted cells during the differentiation process or to enrich the cells of interest by positive selection.
Markers for ES– or iPS cell–derived cell types:
- Hematopoietic stem and progenitor cells: CD34, CD117, CD133, or Sca-1
- Endothelial and progenitor cells: CD31, CD133, CD34, or Sca-1
- Mesenchymal stromal cells (MSCs): CD105, CD146, CD271, or MSCA-1
- Neural precursors: A2B5, PSA-NCAM, CD133, or CD11b
- Smooth muscle cells: Sca-1
If the cell marker of your interest is not listed, click here to discover how to isolate any cell from any species using our indirect magnetic labeling system.
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| September 2010 |
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