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MACS® Cell Separation—the principle
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> The principle
> Labeling strategies
> Separation strategies
A poster depicting MACS Cell Separation Strategies can be downloaded here. The poster is also available in print format, which additionally presents a variety of amazing images of cells separated with MACS Technology - please order your personal copy here.
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Direct magnetic cell labeling
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- Fast way of magnetic labeling
- Requires only one labeling step
- Specific antibody is directly coupled to the magnetic particle
- Reduces the number of washing steps and avoids unnecessary cell loss
- Available for many different surface markers of cells from various species
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Indirect magnetic cell labeling
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- For virtually any cell type from virtually any species
- If no direct MicroBeads for the cell type of interest are available
- Labeling of the cells with a primary antibody of choice followed by magnetic labeling with Anti-Fluorochrome, Anti-Biotin, Streptavidin, or Anti-Immunoglobulin MicroBeads
- For virtually any monoclonal or polyclonal primary antibody
- Method of choice for magnetic separation according to dimly expressed antigens
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