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MACS® Cell Separation—the principle
> The principle
> Labeling strategies

> Separation strategies
 
A poster depicting MACS Cell Separation Strategies can be downloaded here.
The poster is also available in print format, which additionally  presents a variety of amazing images of cells separated with MACS Technology - please order your personal copy here.
Labeling strategies
Direct magnetic cell labeling 
Direct magnetic cell labeling
  • Fast way of magnetic labeling
  • Requires only one labeling step
  • Specific antibody is directly coupled to the magnetic particle
  • Reduces the number of washing steps and avoids unnecessary cell loss
  • Available for many different surface markers of cells from various species
Indirect magnetic cell labeling 
Indirect magnetic cell labeling
  • For virtually any cell type from virtually any species
  • If no direct MicroBeads for the cell type of interest are available
  • Labeling of the cells with a primary antibody of choice followed by magnetic labeling with Anti-Fluorochrome, Anti-Biotin, Streptavidin, or Anti-Immunoglobulin MicroBeads
  • For virtually any monoclonal or polyclonal primary antibody
  • Method of choice for magnetic separation according to dimly expressed antigens
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