| Embryonic stem cells and induced pluripotent stem cells |
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Embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells have the capacity to self-renew and to differentiate into all cell types of the body. They promise an essentially unlimited supply of specific cell types for basic research, drug testing, and possibly for future transplantation therapies.To investigate these cells it is crucial to use homogenous cell populations. MACS® Technology enables the isolation of numerous particular cell types and subsets at high purity.
Hematopoietic stem cells (HSCs) can be isolated from differentiated ESCs by using, for example, the following cell surface markers:
• CD34
• CD43
• CD133
Endothelial progenitor cells (EPCs) can be isolated from ESCs by using, for example:
• CD34
• CD31
Miltenyi Biotec also offers numerous products for the enrichment of various neural cell types that can be derived from ESCs, such as:
• CD133+ neural stem cells,
• PSA-NCAM+ neuronal-restricted precursors,
• A2B5+ glial-restricted precursors,
• CD11b+ microglial cells,
• CD271 (LNGFR, p75 NTR)+ Schwann cells and motor neurons.
Removal of feeder cells – MACS Technology enables the separation of ESCs or iPS cells from feeder cells.
Enrichment of undifferentiated, pluripotent ESCs – MACS Cell Separation also allows the enrichment of pluripotent stem cells, either by depletion of differentiated cells or by positive selection according to the expression of pluripotency-associated markers, such as SSEA-3, SSEA-4, TRA-1-60, or TRA-1-81. This is achieved by using specific primary antibodies of choice in combination with MicroBeads for indirect magnetic labeling.
Efficient generation of iPS cells – The fast magnetic isolation of fully reprogrammed cells with high expression levels of SSEA-3+ may enhance the generation of iPS cells.
Please refer to the flyer for ESC and iPS cell research to get more information.
For more information on products for research on mouse ESCs and iPS cells, click here. |
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