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Miltenyi Biotec Miltenyi Biotec Cytokine detection | for human cells | Cytokine Secretion Assays
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MACSQuant® Analyzer
Antibodies
navigationCytokine detection
Miltenyi Biotec
for human cellsfor human cellsfor human cells
Cytokine Secretion AssaysCytokine Secretion AssaysCytokine Secretion AssaysCytokine Secretion Assays
Kits for intracellular cytokine stainingKits for intracellular cytokine stainingKits for intracellular cytokine stainingKits for intracellular cytokine staining
Cytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodiesCytokine detection antibodies
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for non-human primate cellsfor non-human primate cellsfor non-human primate cells
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for mouse cellsfor mouse cellsfor mouse cells
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Kits for cell detection and analysis
Tumor cell analysis
Immunofluorescent amplification (FASER Kits)
Inside Stain Kit
Staining of apoptotic and dead cells
Compensation reagents
Antibody biotinylation
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MACS® Cytokine Secretion Assays
MACS® Cytokine Secretion Assays
MACS® Cytokine Secretion Assays are designed for highly sensitive detection as well as optional enrichment of viable cytokine-secreting cells. This unique technology allows:
  • Detection of secreted cytokines on single-cell level
  • Simultaneous phenotyping of cytokine-secreting cells for more detailed information on subsets and activation status
  • Detection of coexpression of two different cytokines or simultaneous staining with peptide-MHC tetramers for functional analysis of the cells
  • Optional magnetic enrichment of viable cytokine-secreting cells for enhanced sensitivity of one antigen-specific T cell per million cells or downstream applications (MACS® Secretion Assays - Enrichment and Detection Kits)

For polyclonal activation of human T cells, Miltenyi Biotec offers CytoStim and the T Cell Activation/Expansion Kit. Antigen-specific stimulation can be achieved with the CMV pp65 - Recombinant Protein or the PepTivator® Peptide Pools for specific CD4+ and CD8+ T cell stimulation.
Details and background information
Cytokine analysis – product list [PDF, 184 KB]
IFN-α Secretion Assay – Detection Kit
ProductApplicationsorder no.
IFN-α Secretion Assay – Detection Kit (PE), humanDetection of human IFN-α–secreting cells130-094-161
IFN-γ Secretion Assays – Detection Kits
ProductApplicationsorder no.
IFN-γ Secretion Assay – Detection Kit (PE), humanDetection of human IFN-γ-secreting cells130-054-202
IFN-γ Secretion Assay – Detection Kit (FITC), humanDetection of human IFN-γ-secreting cells130-090-433
IFN-γ Secretion Assay – Detection Kit (APC), humanDetection of human IFN-γ-secreting cells130-090-762
IL-2 Secretion Assays – Detection Kits
ProductApplicationsorder no.
IL-2 Secretion Assay – Detection Kit (PE), humanDetection of human IL-2-secreting cells130-090-487
IL-2 Secretion Assay – Detection Kit (APC), humanDetection of human IL-2-secreting cells130-090-763
IL-4 Secretion Assay - Detection Kit
ProductApplicationsorder no.
IL-4 Secretion Assay – Detection Kit (PE), humanDetection of human IL-4-secreting cells130-054-102
IL-5 Secretion Assays – Detection Kits
ProductApplicationsorder no.
IL-5 Secretion Assay – Detection Kit (PE), humanDetection of human IL-5-secreting cells130-091-623
IL-5 Secretion Assay – Detection Kit (APC), humanDetection of human IL-5-secreting cells130-091-624
IL-10 Secretion Assays – Detection Kits
ProductApplicationsorder no.
IL-10 Secretion Assay – Detection Kit (PE), humanDetection of human IL-10-secreting cells130-090-434
IL-10 Secretion Assay – Detection Kit (APC), humanDetection of human IL-10-secreting cells130-090-761
IL-12 Secretion Assay – Detection Kit
ProductApplicationsorder no.
IL-12 Secretion Assay – Detection Kit (PE), humanDetection of human IL-12-secreting cells130-092-124
IL-13 Secretion Assay – Detection Kit
ProductApplicationsorder no.
IL-13 Secretion Assay – Detection Kit (PE), humanDetection of human IL-13-secreting cells130-093-479
IL-17 Secretion Assay – Detection Kits
ProductApplicationsorder no.
IL-17 Secretion Assay – Detection Kit (PE), humanDetection of viable human IL-17–secreting leukocytes130-094-537
IL-17 Secretion Assay – Detection Kit (APC), humanDetection of viable human IL-17–secreting leukocytes130-094-536
TNF-α Secretion Assays – Detection Kits
ProductApplicationsorder no.
TNF-α Secretion Assay – Detection Kit (PE), humanDetection of human TNF-α-secreting cells130-091-268
TNF-α Secretion Assay – Detection Kit (APC), humanDetection of human TNF-α-secreting cells130-091-267
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