| Description |
Genotyping and mRNA expression profiling of KIR genes
The KIR Typing Kit allows the detection of human KIR (killer cell immunoglobulin-like receptor) genes on genomic DNA or mRNA level.
Accurate primer design
The presence or absence of KIR genes is analyzed by PCR technology using sequence-specific primers (SSPs). Carefully designed SSPs enable the detection of all known 15 human KIR genes plus two pseudo genes.
Ready to use enzyme mix and reagents
Lyophilized enzyme mix, complete with Taq DNA Polymerase, is pre-distributed in each well of the 96-well PCR plate. The Resuspension Buffer is simply mixed with the template and dispensed into the wells of the PCR plate. The PCR products are ready for immediate analysis by electrophoresis due to the integrated gel loading buffer.
Internal positive control
In addition to the KIR-specific primers, the wells contain positive control primers. Thus, two fragments per well can be amplified.
This product is for research use only and not for diagnostic or therapeutic use. |
| Applications |
KIRs can be found on natural killer (NK) cells which play a critical role in the innate immune response to viral infection and tumor cell lysis. Furthermore, NK cells have been implicated in the suppression of graft versus host disease, promotion of bone marrow engraftment, and mediation of a graft versus leukemia effect. The KIRs exist in various isoforms. The inhibitory KIRs interact with specific HLA class I molecules, predominantly HLA-C, on target cells. Subsequently, the NK cells become inhibited.
Not all KIR genes are present in every human individual. Further heterogeneity exists at the transcriptional level. Different subsets of NK cells express different KIRs, even within one individual.
The KIR Typing Kit allows the investigation of the KIR genotype on the DNA level and its expression on mRNA level. |
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| Figure 1 |
| KIR Typing Kit with ready-to-use solutions, lyophilized enzyme mix in a 96-well PCR plate, and protocol. |
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| Figure 2 |
KIR genotyping
Gel fractionation of PCR products obtained from genomic DNA isolated from whole blood. Lanes 1–5, 7, 9–12, 14–16 and 18–19 show positive KIR (+). Lanes 6, 8, and 13 are negative for KIR (–). |
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| All lanes show in addition positive control bands (400 bp). Lane 20 shows the genomic DNA control band at 260 bp, lane 21 the positive control, and lane 22 the negative control. M: DNA marker |
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| Figure 3 |
mRNA expression profiling of KIR genes
Gel fractionation of PCR products obtained from mRNA-derived cDNA isolated from 5 mL whole blood. Lanes 1–4, 9–12 and 14–15 show positive KIR bands (+), whereas lanes 5–8, 13, and 16–19 are negative for KIR (–). |
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| In addition, all lanes include positive control bands (400 bp). Lane 20 shows no genomic DNA control band at 260 bp; thus, there is no genomic DNA contamination. M: DNA marker |
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| Figure 4 |
Gene name and allele specificity
The KIR Typing Kit identifies the presence or absence of the following KIR genes and variants: |
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