| Description |
| CD45 MicroBeads are used for the depletion or enrichment of leukocytes from peripheral blood, lymphoid tissue, tumor tissue, or non-hematopoietic tissue. CD45 is expressed on all cells of hematopoietic origin except erythrocytes and platelets and their precursor cells. |
| Applications |
| CD45 MicroBeads have been used to enrich non-hematopoietic tumor cells from peripheral blood, bone marrow, or lymph nodes of patients by depletion of CD45+ leukocytes.1–4 |
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| Figure 1 |
| A cytokeratin-positive cell was enriched from bone marrow of a patient with renal carcinoma by depletion of leukocytes using CD45 MicroBeads. Cells were stained using a conventional APAAP method followed by staining with hematoxylin. (Courtesy of Dr. Bilkenroth, Dept. Pathol. and Dr. Meye, Dept. Urol., Univ. Halle, Germany.) |
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| Figure 2 |
| Enrichment of tumor cells from a mixture of PBMCs and cells of the breast cancer cell line SK-BR3 using CD45 MicroBeads. |
| A: Cell mixture before separation (7Ă—106 PBMCs were spiked with 20,000 tumor cells) |
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| B: Negative fraction with enriched breast cancer cells |
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