| Description |
The Anti-Ly-6G MicroBead Kit was developed for positive selection or depletion of mouse neutrophils from single-cell suspensions of lymphoid tissues.
Ly-6G is highly expressed on neutrophils, at lower levels on a subset of eosinophils, and transiently during developmental stages on monocytes. For separation, neutrophils are indirectly magnetically labeled with Anti- Ly-6G-Biotin and Anti-Biotin-MicroBeads. The Anti-Ly-6G MicroBead Kit has been optimized for isolation of Ly-6Ghigh-expressing cells. |
| Applications |
| Ly-6G+ cells isolated with the Anti-Ly-6G MicroBead Kit are suitable for in vitro analysis, coculture or adoptive transfer experiments. |
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| Figure 1 |
| Ly-6G(1A8)+ cells were isolated from mouse spleen cells by using the Anti-Ly-6G MicroBead Kit, two MS Columns and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-Gr-1-PE (# 130-091-932; clone RB6-8C5)) and CD11b-FITC (# 130-081-201). Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| A: Spleen cells before separation |
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| B: Spleen cells depleted of Ly-6G+ cells |
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| C: Isolated Ly-6G+ neutrophils |
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