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MACS® Cell Separation Columns
MACS® Cell Separation Columns
MACS® Cell Separation Columns were developed for the gentle separation of a wide range of cells. When MACS Columns are placed in a MACS Separator – a strong permanent magnet – the MACS Column matrix provides a magnetic field, strong enough to retain cells labeled with minimal amounts of MACS MicroBeads.

MS Columns, LS Columns, and XS Columns are optimized for positive selection of cells. They can also be used for depletion if the magnetic labeling is strong.

Large Cell Columns were designed for positive selection of large human or animal cells (e.g. megakaryocytes) and have a larger pore size compared to MS Columns.

LD Columns, as well as CS Columns and D Columns are recommended for depletion of unwanted cells. Highest depletion efficiency is obtained with these column types, even if the magnetic labeling of the cells – due to dim antigen expression – is weak.

The Whole Blood Column Kit is suited for manual separation of leukocyte subsets directly from human whole blood.

autoMACS Columns are designed for use with the autoMACSâ„¢ Separator and the autoMACSâ„¢Pro Separator.

Which column should be used for a certain cell separation reagent? Please refer to the respective reagent data sheet, which includes information on the recommended MACS Columns.
Details and background information
Column_Decision_Flyer [PDF, 1 MB]
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