| Description |
Anti-DC (OX62) MicroBeads have been developed for the positive selection or depletion of OX62+ dendritic cells (DCs) from blood, lymphoid, and non-lymphoid tissue.
The monoclonal antibody OX62 recognizes the integrin αE2 subunit (molecular mass: 150 kDa). OX62 is expressed on the
CD4+ and the CD4- subset of dendritic cells but not on plasmacytoid dendritic cells in lymphoid tissue or on epidermal Langerhans cells. OX62 is also found on a subpopulation of epidermal TCRα/β+ or TCRγ/δ+ T cells and on a subset of folliculo-stellate-like (S100+) cells in the anterior pituitary. It is not expressed on macrophages. |
| Applications |
| Isolated OX62+ dendritic cells were used, for example, for analysis of the differential phenotype, function, and migration of dendritic cell subsets.1 |
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| Figure 1 |
| Isolation of dendritic cells from rat spleen using Anti-DC (OX62) MicroBeads, a MiniMACS™ Separator, and an MS Column. Isolated cells were stained with Anti-MHC class II (OX6)-FITC, CD4-PE, and Anti-DC (OX62)-APC. |
| A: Spleen cells before separation |
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| B: Isolated dendritic cells |
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| C: Isolated dendritic cells |
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| Figure 2 |
| Isolated OX62+ dendritic cells from rat spleen, May-GrĂĽnwald/Giemsa stained. (Courtesy of Dr. R. Josien, Nantes, France.) |
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